Journal: Cancer Discovery

Article Title: Spatially Segregated Macrophage Populations Predict Distinct Outcomes in Colon Cancer

doi: 10.1158/2159-8290.CD-23-1300

Figure Lengend Snippet: SPP1 TAMs are enriched in hypoxic and necrotic tumor areas and NLRP3 TAMs activate NLRP3 inflammasome in the TME. A, Immunohistochemical image shows NLRP3 TRMs surrounded by neutrophils (arrowheads). B, Immunohistochemical image shows SPP1 TRMs surrounded by karyorrhectic debris in necrotic material (arrowheads). C, Volcano plot shows differential gene expression between scRNA transcriptomes of SPP1 TAMs and NLRP3 TAMs. D, Dotplot of average expression of genes associated with neutrophil chemoattraction, lipid metabolism and phagocytosis across scRNA macrophage populations. E, Dotplot shows the annotation of tumor (green) and necrotic (brown) areas (top left) and normalized expression of SPP1 (top right) and NLRP3 (bottom right) on the 10× Visium FFPE Human Breast Cancer sample, and barplot shows normalized log 2 SPP1 expression in tumor and necrosis regions (bottom left). F, IF shows a representative breast cancer region stained with NLRP3, CD68, Calprotectin (CPTN), and DAPI. Scale bar of 10 μm is identical for all close-up images. G, Quantification of the number of neutrophils present on nine breast cancer 1.5 mm 2 tissue regions stratified by whether they contained diffuse NLRP3 (three regions) or NLRP3 specks (six regions). P value was computed using a two-sided Wilcoxon’s rank-sum test. H, Schematic of a possible mechanism through which NLRP3 TAMs can contribute to the recruitment of neutrophils in the TME. I, Sina plots show marker protein expression profiled using CosMx SMI 64-plex Human Immuno-Oncology Protein Panel.

Article Snippet: For the analysis in and Supplementary Fig. S7D, CosMx protein data were produced by NanoString company as a service using the CosMx SMI 64-plex Human Immuno-Oncology Protein Panel.

Techniques: Immunohistochemical staining, Expressing, Staining, Marker